The genus parapoxvirus includes four members bovine papular stomatitis virus bpsv pseudocowpox virus pcpv orf virus orfv and parapoxvirus of red deer in new zealand pvnz a set of primers for polymerase chain reaction pcr was designed to detect viral dna from cells infected with each of the four parapoxviruses. Polymerase chain reaction for laboratory diagnosis of orf virus infections the sheep and goat industries but also threatens human health for viral infectious diseases fast and effective . The rapid advances in virus gene cloning and sequencing coupled with the rapid detection of viruses by polymerase chain reaction pcr have revolutionized clinical diagnostic virology the rapid detection of viruses and viral genomes and messenger rna may make it possible to detect viruses in clinical specimens and transplanted organs. Journal of virological methods 25 1989 179 188 179 eisevier virmet 00904 the polymerase chain reaction a review of the practical limitations for human immunodeficiency virus diagnosis jp clewley virus reference laboratory central public health laboratory london uk accepted 23 march 1989 summary the polymerase chain reaction pcr is a powerful method for the in vitro am . A comparative study of virus isolation polymerase chain reaction and antigen detection in children infected with human virus of mothers immunodeficiency anne krivine md ahmed yakudima ms mireille le may md victor pena cruz md alice s huang phd and kenneth mclntosh md from the division of infectious diseases childrens hospital the departments of pediatrics and of microbiology
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